Team:University of Ottawa/11 July 2008

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Contents

Today in the Lab

Matt

PTP2 Digestion
  • Ran two digestion's of the PTP2 in parallel with one another: AatII/NcoI, EcoRV/NcoI. Neither Gel gave proper band sizes.
  • PCR confirmation
  • PCR confirmation of 97 plasmid showed up nicely on the gel, confirming that the integration was a success.
  • Chris

    Confirmation of AtCRE
  • ran the 1% gel at 80 V for about forty minutes
  • only primer bands showed after this time period
  • it was suggested that phusion polymerase may not have been added before PCR, so 1 uL of phusion was added to the samples and phusion PCR was re-initiated
  • following PCR, the results were run on a gel with the same results as above.