Team:University of Ottawa/15 July 2008

From 2008.igem.org

(Difference between revisions)
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==Today on the Lab==
==Today on the Lab==
'''Tammy'''
'''Tammy'''
-
:<li>Transformation of XL10 Competent E. coli cells with pDR197:AtCKX2
+
'''Transformation of XL10 Competent E. coli cells with pDR197:AtCKX2'''
:<li>1 uL Pure pDR197:AtCKX2
:<li>1 uL Pure pDR197:AtCKX2
:<li>1 ul 1:10 dilution pDR197:AtCKX2
:<li>1 ul 1:10 dilution pDR197:AtCKX2
Line 10: Line 10:
:<li>3 LB Ampicillin (50 ug/mL) Plates
:<li>3 LB Ampicillin (50 ug/mL) Plates
:<li>Began Incubation of transformed cells at 1:50 pm.
:<li>Began Incubation of transformed cells at 1:50 pm.
 +
'''Matt'''
 +
'''Inoculation of 97 - BY4742 cells'''
 +
:<li> I inoculated the 97 BY4742 cells once again for glycerol stock tomorrow
 +
'''PCR confirmation'''
 +
:<li> Second PCR confirmation with F58, F59 was performed and gave the correct band but also gave another weird band that I don't know what it is.
 +
'''Digestion of PTP2'''
 +
<li> A digestion was performed on the PTP2 amplification product to create sticky ends using BamHI and xhoI and I left it in the PCR machine overnight.

Revision as of 19:26, 16 July 2008

Contents

Today on the Lab

Tammy Transformation of XL10 Competent E. coli cells with pDR197:AtCKX2

  • 1 uL Pure pDR197:AtCKX2
  • 1 ul 1:10 dilution pDR197:AtCKX2
  • 1 ul 1:100 dilution pDR197:AtCKX2
  • 100 ul XL10 E.Coli per reaction
  • 900 ul LB Broth per reaction
  • 3 LB Ampicillin (50 ug/mL) Plates
  • Began Incubation of transformed cells at 1:50 pm.
  • Matt Inoculation of 97 - BY4742 cells

  • I inoculated the 97 BY4742 cells once again for glycerol stock tomorrow
  • PCR confirmation

  • Second PCR confirmation with F58, F59 was performed and gave the correct band but also gave another weird band that I don't know what it is.
  • Digestion of PTP2

  • A digestion was performed on the PTP2 amplification product to create sticky ends using BamHI and xhoI and I left it in the PCR machine overnight.