Team:University of Ottawa/15 July 2008

From 2008.igem.org

(Difference between revisions)
(Today on the Lab)
(Today on the Lab)
Line 13: Line 13:
:'''Inoculation of 97 - BY4742 cells'''
:'''Inoculation of 97 - BY4742 cells'''
:<li> I inoculated the 97 BY4742 cells once again for glycerol stock tomorrow
:<li> I inoculated the 97 BY4742 cells once again for glycerol stock tomorrow
-
'''PCR confirmation'''
+
:'''PCR confirmation'''
:<li> Second PCR confirmation with F58, F59 was performed and gave the correct band but also gave another weird band that I don't know what it is.
:<li> Second PCR confirmation with F58, F59 was performed and gave the correct band but also gave another weird band that I don't know what it is.
-
'''Digestion of PTP2'''
+
:'''Digestion of PTP2'''
:<li> A digestion was performed on the PTP2 amplification product to create sticky ends using BamHI and xhoI and I left it in the PCR machine overnight.
:<li> A digestion was performed on the PTP2 amplification product to create sticky ends using BamHI and xhoI and I left it in the PCR machine overnight.

Revision as of 19:27, 16 July 2008

Contents

Today on the Lab

Tammy

Transformation of XL10 Competent E. coli cells with pDR197:AtCKX2
  • 1 uL Pure pDR197:AtCKX2
  • 1 ul 1:10 dilution pDR197:AtCKX2
  • 1 ul 1:100 dilution pDR197:AtCKX2
  • 100 ul XL10 E.Coli per reaction
  • 900 ul LB Broth per reaction
  • 3 LB Ampicillin (50 ug/mL) Plates
  • Began Incubation of transformed cells at 1:50 pm.
  • Matt

    Inoculation of 97 - BY4742 cells
  • I inoculated the 97 BY4742 cells once again for glycerol stock tomorrow
  • PCR confirmation
  • Second PCR confirmation with F58, F59 was performed and gave the correct band but also gave another weird band that I don't know what it is.
  • Digestion of PTP2
  • A digestion was performed on the PTP2 amplification product to create sticky ends using BamHI and xhoI and I left it in the PCR machine overnight.