"
Team:University of Ottawa/17 July 2008
From 2008.igem.org
(Difference between revisions)
(New page: ==Today in the Lab== '''Chris''' :'''Purification of AtCRE''' ::<li> used PCR clean up kit to purify AtCRE sample :'''Tranformation of Competent Cells''' ::<li> followed transformation of...) |
|||
| Line 1: | Line 1: | ||
| + | __TOC___ | ||
==Today in the Lab== | ==Today in the Lab== | ||
| - | |||
'''Chris''' | '''Chris''' | ||
:'''Purification of AtCRE''' | :'''Purification of AtCRE''' | ||
| Line 7: | Line 7: | ||
::<li> followed transformation of competent cells protocol to insert AtCRE plasmid into preprepared competent E. coli cells. | ::<li> followed transformation of competent cells protocol to insert AtCRE plasmid into preprepared competent E. coli cells. | ||
::<li> allowed cells to incubate overnight at 37 C | ::<li> allowed cells to incubate overnight at 37 C | ||
| + | '''Matt''' | ||
| + | :'''Ligation''' | ||
| + | ::<li> As suggested by Dan the ligation was spiked with 1 ul ATP. | ||
| + | ::<li> I then performed a PCR cleanup of the Ligation product in order to purify it for transformation into competent cells. | ||
| + | :'''Transformation''' | ||
| + | ::<li> A transformation was performed on PTP2 ligation product - I then left the streaked plates to incubate for tomorrow. | ||
| + | ::<li> A glycerol stock was finally made of the BY4642 yeast strain int. DQ232597. | ||
Revision as of 18:35, 18 July 2008
Contents |
_
Today in the Lab
Chris
- Purification of AtCRE
- used PCR clean up kit to purify AtCRE sample
- Tranformation of Competent Cells
- followed transformation of competent cells protocol to insert AtCRE plasmid into preprepared competent E. coli cells.
- allowed cells to incubate overnight at 37 C
Matt
- Ligation
- As suggested by Dan the ligation was spiked with 1 ul ATP.
- I then performed a PCR cleanup of the Ligation product in order to purify it for transformation into competent cells.
- Transformation
- A transformation was performed on PTP2 ligation product - I then left the streaked plates to incubate for tomorrow.
- A glycerol stock was finally made of the BY4642 yeast strain int. DQ232597.
