Team:University of Ottawa/25 July 2008

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Dan

Miniprep
  • Minipreped the 6 colonies, obatined good concentrations however gel confirmation revealed that transformation was unsuccessful.
  • Gel Extraction
  • Performed gel extraction of T123 amplification product from July 24
  • To much DNA was inserted into each well, will have to do PCR again
  • PCR of 0A and 0B
  • PCR of constructs 0A and 0B was performed, producing lots of DNA.


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