Team:University of Sheffield

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!align="center"|[[Team:University_of_Sheffield |Introduction]]
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!align="center"|[[Team:University_of_Sheffield |Home]]
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!align="center"|[[Team:University_of_Sheffield /Project|Our project]]
!align="center"|[[Team:University_of_Sheffield /Project|Our project]]
!align="center"|[[Team:University_of_Sheffield /Modelling|Modelling]]
!align="center"|[[Team:University_of_Sheffield /Modelling|Modelling]]
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!align="center"|[[Team:University_of_Sheffield /Wet Lab|Wet Lab]]
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!align="center"|[[Team:University_of_Sheffield /Lab Books| Our team]]
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!align="center"|[[Team:University_of_Sheffield /Administration|Administration]]
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!align="center"|[[Team:University_of_Sheffield /Misc| Miscellaneous]]
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=On a Mission for Fluorescence...=
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[[image:GFP_structure.jpg|250px|thumb|right|Structure of GFP courtesy of [http://www.tsienlab.ucsd.edu Tsien Lab]]]
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|<H2>On a mission for luminescence</H2>
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<div class="floatright" style="width:200px">[[image:GFP_E.coli.jpg|200px|E.coli transformed with GFP plasmid. How exciting!]]<br>''<p>Glowing E.coli</p>''</div>
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<p>The aim of this year’s project is to engineer a biological machine which can sense cholera contamination in water, and rapidly test if water is potable. Technically, to achieve this easy way of testing water contamination the team is planning to apply quorum sensing. [http://en.wikipedia.org/wiki/Quorum_sensing Quorum sensing] bacteria have the ability to both secrete signaling molecules and receive them. When signaling molecule is present in the environment it binds to the receptor and activates a particular action within the organism. [https://2008.igem.org/Team:University_of_Sheffield_/Project More]. </p>
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Hygiene related diseases have an undeniably huge impact on the world we live in, where many deaths are preventable if sanitary conditions could be improved. This issue is not limited to developing countries: outbursts of diseases caused by water contamination still occur all around the world including the most developed regions. Efficient testing of water is an essential part of preventing these incidents, especially after natural disasters. It needs to be quick, easy enough for anyone to use, cheap and reusable. This was the idea behind the University of Sheffield’s project in its first entry into iGEM.
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By manipulating a pathogen's quorum sensing system and hijacking a pathway already in E.coli, we wanted to build an E.coli cell that could detect ''Vibrio cholerae'' and produce a GFP glow. This would provide the criteria for a good water tester, and hopefully a springboard into the development of other pathogen-detecting E.coli.
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|<H2>The University of Sheffield</H2>
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Adding further depth to the project, our sensor was to be focused around the building of a fusion receptor protein. This would consist of the ‘sensing part’ of the pathogen’s receptor (which detects its own quorum signals) fused to the response regulator of the chosen E.coli receptor. GFP fused downstream of the E.coli receptor’s target genes mean that pathogen present = glow!
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<div class="floatright" style="width:350px">[[image:Firth_Court.png‎|350px|How lovely]]<br>''<p>Firth Court, Home of Molecular Biology</p>''</div>
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You can read more about our project [[Team:University_of_Sheffield_/Project | here]], or use the navigation bar above.
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<p>Our University is one of the six original [http://en.wikipedia.org/wiki/Red_Brick Red Brick Universities], and is therefore one of the best in the country. <br><br>
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=Sheffield University=
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Its Molecular Biology and Biotechnology department is No.1 in the University, with the Engineering Department not far behind. Its from these departments our team members are proud to originate.<br><br>
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[http://www.shef.ac.uk/ The University of Sheffield] is one of the top 'Russel Group' universities in Great Britain, with very highly ranked  Molecular Biology and Biotechnology and Engineering departments - which is handy for iGEM!  Alternatively, if you fancy a visit, we are home to the best Students Union in the country!
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The University of Sheffield put its emphasis in the scientific departments on research-led teaching, including a £30m refurb on Firth Court (pictured)</p>
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[[Image:Firth_court.png|center|250px|Firth Court, Molecular Biology and Biotechnology department]]
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<div class="floatleft" style="width:200px">[[image:Union_on_concourse.png|200px|Just the front!]]<br>''<p>The Union entrance</p>''</div>
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=Sponsors=
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[[Image:ChELSI_Logo_small.png|thumb|left|100x100px|[http://smic.group.shef.ac.uk/04b_ChELSI.html ChELSI]]]
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[[Image:Prof_Robert_Poole_logo.png|thumb|left|100x100px|[http://www.shef.ac.uk/mbb/staff/poole/poolelab.html Professor Robert Poole]]]
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[[Image:Idtdna-logogif.gif|thumb|left|100x100px|[http://eu.idtdna.com/Home/Home.aspx IDT DNA]]]
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[[Image:Biofusion_logo.png|thumb|left|100x100px|[http://www.fusionip.co.uk/ Fusion IP]]]
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<p>The University Union is one of the biggest in the country, and has just won the national award for Best Union 2nd year running!</p>
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[[Image:IChemE.jpg‎|thumb|left|100x100px|[http://www.icheme.org/enetwork/MainFrameset.asp?AreaID=171 Institute of Chemical Engineering]]]
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[[Image:Sheffield_union_logo.PNG|thumb|left|100x100px|[http://www.shef.ac.uk/union/ Sheffield University Union of Students]]]
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|<H2>Sponsors</H2>
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||<div class="floatmiddle" style="width:300px">[[image: ChELSI_Logo_small.png|300px|Click to go to their website]]<br>''<p> Chelsi Group, University of Sheffield</p>''</div>
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|<div class="floatmiddle" style="width:200px">[[image: Prof_Robert_Poole_logo.png|200px|Click to go to his website]]<br>''<p> Prof Robert Poole<br>MBB Department<br>University of Sheffield</p>''</div>
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|<div class="floatmiddle" style="width:300px">[[image:Idtdna-logogif.gif|300px|Click to go to their website]]<br>''<p>IDT DNA Oligo and DNA synthesis</p>''</div>
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|<div class="floatmiddle" style="width:200px">[[image: Biofusion_logo.png|150px|Click to go to their website]]<br>''<p>Fusion IP Group</p>''</div>
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|<div class="floatmiddle" style="width:300px">[[image:IChemE.jpg‎|300px|Click to go to their website]]<br>''<p>iChemE</p>''</div>
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|<div class="floatmiddle" style="width:300px">[[image:Sheffield_union_logo.PNG|300px|Click to go to the website]]<br>''<p>Sheffield University Union</p>''</div>
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Latest revision as of 14:35, 29 October 2008

UniShefBanner.jpg


Introduction Our project Modelling Wet Lab Our team Timetable Miscellaneous


On a Mission for Fluorescence...

Structure of GFP courtesy of Tsien Lab

Hygiene related diseases have an undeniably huge impact on the world we live in, where many deaths are preventable if sanitary conditions could be improved. This issue is not limited to developing countries: outbursts of diseases caused by water contamination still occur all around the world including the most developed regions. Efficient testing of water is an essential part of preventing these incidents, especially after natural disasters. It needs to be quick, easy enough for anyone to use, cheap and reusable. This was the idea behind the University of Sheffield’s project in its first entry into iGEM.

By manipulating a pathogen's quorum sensing system and hijacking a pathway already in E.coli, we wanted to build an E.coli cell that could detect Vibrio cholerae and produce a GFP glow. This would provide the criteria for a good water tester, and hopefully a springboard into the development of other pathogen-detecting E.coli. Adding further depth to the project, our sensor was to be focused around the building of a fusion receptor protein. This would consist of the ‘sensing part’ of the pathogen’s receptor (which detects its own quorum signals) fused to the response regulator of the chosen E.coli receptor. GFP fused downstream of the E.coli receptor’s target genes mean that pathogen present = glow!

You can read more about our project here, or use the navigation bar above.

Sheffield University

The University of Sheffield is one of the top 'Russel Group' universities in Great Britain, with very highly ranked Molecular Biology and Biotechnology and Engineering departments - which is handy for iGEM! Alternatively, if you fancy a visit, we are home to the best Students Union in the country!

Firth Court, Molecular Biology and Biotechnology department

Sponsors

ChELSI
Professor Robert Poole
IDT DNA
Fusion IP
Institute of Chemical Engineering
Sheffield University Union of Students
Retrieved from "http://2008.igem.org/Team:University_of_Sheffield"