Team:University of Washington/Parts

From 2008.igem.org

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!align="center"; style="border-color:#6b0c6a"|[[Team:University_of_Washington|<font color="gold">Home</font>]]
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===Parts===
===Parts===
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Sent:
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{| style="text-align:center;color:Black;background-color:Gold;border-color:#6b0c6a;" border=2 cellpadding=5  cellspacing=2
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!Parts!!Vector plasmid!!Resistance!!Description
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|BBa_K109200||pSB1AC3||amp/kan||align="left"|Promoter from Michael Elowitz’s group activatable by AraC/arabinose and repressible by TetR.
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|BBa_K109201||pSB3K3||kan||align="left"|K109201 promoter in front of E0240 GFP
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|BBa_K109202||pSB3K3||kan||align="left"|K109201 promoter in front of LuxR (I0462)
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|-
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|BBa_K109550||pSB1A3||amp||align="left"|Conjugative master regulator KorA
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|-
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|BBa_K109551||pSB1A3||amp||align="left"|Conjugative factor TrbA
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|-
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|BBa_K109702||pSB3K3||kan||align="left"|Lac promoter (R0010) in front of LuxR (I0462)
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|}
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On process:
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{| style="text-align:left;color:Black;background-color:Gold;border-color:#6b0c6a;" border=2 cellpadding=5  cellspacing=2
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|K109064||RBS measurement construct w/ mRFP (J61104 + E1010)
|K109064||RBS measurement construct w/ mRFP (J61104 + E1010)
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|K109200||Promoter with AraC and TetR regulators (Elowitz)
 
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|K109201||Reporter GFP(E0240) under control of AraC and TetR(K109200)
 
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|K109202||LuxR(I0462) under control of K109200
 
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|K109702||LacPromoter with LuxR
 
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Latest revision as of 07:54, 27 October 2008

WashingtonColorSeal-21-clip.gif Home The Team The Project Modeling Notebook Protocols Parts Submitted
to the Registry 		Measurement Kit SeToB Safety

Parts

Sent:

PartsVector plasmidResistanceDescription
BBa_K109200pSB1AC3amp/kanPromoter from Michael Elowitz’s group activatable by AraC/arabinose and repressible by TetR.
BBa_K109201pSB3K3kanK109201 promoter in front of E0240 GFP
BBa_K109202pSB3K3kanK109201 promoter in front of LuxR (I0462)
BBa_K109550pSB1A3ampConjugative master regulator KorA
BBa_K109551pSB1A3ampConjugative factor TrbA
BBa_K109702pSB3K3kanLac promoter (R0010) in front of LuxR (I0462)


On process:

NameDescription
K109000pAC88, yeast shuttle vector, nonstandard format
K109001pAC99, yeast shuttle vector with LAC insert, nonstandard format
K109002CEN6-ARS4, yeast origin of replication
K109003HIS3, yeast selectable marker
K109004RP4 OriT, origin of transfer
K109005yeast cloning vector (base vector I51020, yeast origin of replication, and yeast selectable marker)
K109006yeast shuttle vector (base vector I51020, yeast origin of replication, yeast selectable marker, origin of transfer, bacterial origin of replication, and bacterial selectable marker)
K109050RBS measurement construct w/ GFP (J61100 + E0040)
K109051RBS measurement construct w/ GFP (J61101 + E0040)
K109052RBS measurement construct w/ GFP (J61102 + E0040)
K109053RBS measurement construct w/ GFP (J61103 + E0040)
K109054RBS measurement construct w/ GFP (J61104 + E0040)
K109060RBS measurement construct w/ mRFP (J61100 + E1010)
K109061RBS measurement construct w/ mRFP (J61101 + E1010)
K109062RBS measurement construct w/ mRFP (J61102 + E1010)
K109063RBS measurement construct w/ mRFP (J61103 + E1010)
K109064RBS measurement construct w/ mRFP (J61104 + E1010)
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