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Team:University of Washington/Protocols
From 2008.igem.org
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5. Decant supernatant into filter cartridge<br\> | 5. Decant supernatant into filter cartridge<br\> | ||
6. Spin 1 min.<br\> | 6. Spin 1 min.<br\> | ||
| - | + | ·empty collection tube<br\> | |
7. Add 750μL PE wash buffer<br\> | 7. Add 750μL PE wash buffer<br\> | ||
8. Spin 1 min.<br\> | 8. Spin 1 min.<br\> | ||
| - | + | ·empty collection tube<br\> | |
9. Dry spin 2 min.<br\> | 9. Dry spin 2 min.<br\> | ||
10. Put filter cartridge in clean eppendorf tube<br\> | 10. Put filter cartridge in clean eppendorf tube<br\> | ||
11. Add 50μL EB<br\> | 11. Add 50μL EB<br\> | ||
| - | + | ·Centrifuge 2 min. @ 9000 rpm | |
== == | == == | ||
[Team:University_of_Washington] | [Team:University_of_Washington] | ||
Revision as of 21:28, 27 June 2008
Sequencing
·primer 8pmol
·4ul DNA
Mini
Qiagen Kit
1. 250μL P1 resuspend pellet
2. 250μL P2
3. 350μL N3
4. Centrifuge full speed 10 min.
5. Decant supernatant into filter cartridge
6. Spin 1 min.
·empty collection tube
7. Add 750μL PE wash buffer
8. Spin 1 min.
·empty collection tube
9. Dry spin 2 min.
10. Put filter cartridge in clean eppendorf tube
11. Add 50μL EB
·Centrifuge 2 min. @ 9000 rpm
[Team:University_of_Washington]
