Team:Utah State/Parts

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!style="text-align:center; background-color:#003300; border-width:0px; padding:2px;"|[[Team:Utah_State|<font color="#ffffff">Home</font>]]
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!style="text-align:center; background-color:#003300; border-width:0px; padding:2px;"|[[Team:Utah_State/Project|<font color="#ffffff">The Project</font>]]
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!style="text-align:center; background-color:#003300; border-width:0px; padding:2px;"|[[Team:Utah_State/Parts|<font color="#99CCFF">Parts</font>]]
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==BIOBRICKS==
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Nine BioBricks were planned as extracts from the PHB operonFive of these were from the PHB promoter and four were from the CAB gene cassette.  
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''Tell us more about your projectGive us background. Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs)''
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|[[Image:Team.png|right|frame|Your team picture]]
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[[Image:PartsUSU.jpg‎|800px|center]]
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==SUBMITTED BIOBRICKS==
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|align="center"|[[Team:Utah_State | Team Example 2]]
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Three PHB promoter BioBricks were successfully ligated into the pSB1A3 plasmidThese BioBricks were constructed to be tested as promoters for green fluorescent protein to determine their autonomy and effectiveness. These BioBricks are labeled in the BioBrick registry as BBa_K089004, BBa_K089005, and BBa_K089006.
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[[Image:USUBiobrick1.jpg‎|320px]][[Image:USUBiobrick2.jpg‎|320px]][[Image:USUBiobrick3.jpg‎|320px]]
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Miniprepped plasmid DNA from each of the BioBricks were placed into PCR tubes and labeled with their designated BioBrick number.  These were placed in a 50-ml free-standing centrifuge tube and sent to iGEM for further testing and placement in the registry.
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[[Image:Submitted_BioBricks_2.JPG‎|320px]][[Image:Submitted_BioBricks_USU‎.JPG|320px]][[Image:Trent_Holding_BioBricks‎.JPG|320px]]
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==BIOBRICKS IN PROGRESS==
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<!--- The Mission, Experiments --->
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The two remaining PHB promoter regions have been successfully amplified using PCR and are in process of ligations and transformations.
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[[Image:USUBiobrick4.jpg‎|450px]][[Image:USUBiobrick5.jpg‎|450px]]
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===Note===
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Two of the CAB cassette genes, phaA and phaB, have been successfully amplified using PCR and are in process of ligation and transformation.  The phaC and phaCAB sequences are in earlier stages. 
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If you choose to include a '''Parts Submitted to the Registry''' page, please list your parts here. This is not necessary but it may be a nice list to keep track of.
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[[Image:BiobrickPHA.jpg‎|440px]]
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[[Image:BiobrickPHAB.jpg‎|440px]]
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[[Image:BiobrickPHAC.jpg‎|430px]]
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[[Image:BiobrickPHCAB.jpg‎|430px]]

Latest revision as of 03:58, 30 October 2008

USUheader2.gif

Home The Team The Project Parts Notebook Protocols Links

BIOBRICKS

Nine BioBricks were planned as extracts from the PHB operon. Five of these were from the PHB promoter and four were from the CAB gene cassette.

PartsUSU.jpg

SUBMITTED BIOBRICKS

Three PHB promoter BioBricks were successfully ligated into the pSB1A3 plasmid. These BioBricks were constructed to be tested as promoters for green fluorescent protein to determine their autonomy and effectiveness. These BioBricks are labeled in the BioBrick registry as BBa_K089004, BBa_K089005, and BBa_K089006.


USUBiobrick1.jpgUSUBiobrick2.jpgUSUBiobrick3.jpg

Miniprepped plasmid DNA from each of the BioBricks were placed into PCR tubes and labeled with their designated BioBrick number. These were placed in a 50-ml free-standing centrifuge tube and sent to iGEM for further testing and placement in the registry.

Submitted BioBricks 2.JPGSubmitted BioBricks USU.JPGTrent Holding BioBricks.JPG

BIOBRICKS IN PROGRESS

The two remaining PHB promoter regions have been successfully amplified using PCR and are in process of ligations and transformations.

USUBiobrick4.jpgUSUBiobrick5.jpg

Two of the CAB cassette genes, phaA and phaB, have been successfully amplified using PCR and are in process of ligation and transformation. The phaC and phaCAB sequences are in earlier stages.

BiobrickPHA.jpg BiobrickPHAB.jpg BiobrickPHAC.jpg BiobrickPHCAB.jpg