Team:Valencia/Notebook/July

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July Mo Tu We Th Fr Sa Su 30  1  2  3  4  5  6  7  8   9 10 11 12 13 14 15  16 17 18 19 20 21 22  23 24 25 26 27 28 29  30 31   1  2  3

August Mo Tu We Th Fr Sa Su 28 29  30 31  1  2  3  4  5   6  7  8  9 10 11 12  13 14 15 16 17 18 19  20 21 22 23 24 25 26  27 28 29 30 31

September Mo Tu We Th Fr Sa Su  1  2   3  4  5  6  7  8  9  10 11 12 13 14 15 16  17 18 19 20 21 22 23  24 25 26 27 28 29 30  1 2 3 4 5

October Mo Tu We Th Fr Sa Su 29 30   1  2  3  4  5  6  7   8 9 10 11 12 13 14  15 16 17 18 19 20 21  22 23 24 25 26 27 28  29 30 31  1  2

November Mo Tu We Th Fr Sa Su 27 28  29 30 31  1  2  3  4   5  6  7  8  9 10 11  12 13 14 15 16 17 18  19 20 21 22 23 24 25  26 27 28 29 30

July 1st

- We prepared some glycerol stock from the four Saccharomyces cerevisiae strains we received from Eduardo Rial.

- We calibrated our calorimeters with hot water.

July 2nd

- We prepared our first SD medium.

- We calibrated our calorimeters with hot water.

July 4th

- We prepared some plates for inoculum.

July 7th

- We prepared some inoculum.

July 8th

- We carried out the pilot experiment.

July 9th

- We characterized culture growth in our calorimeters.

july 10th

- Trying to see if there is basal rate of UCP1...

july 11th

- It looks like our Saccharomyces cerevisiae strains have a basal rate of UCP1... We'll have to repeat the experiment to be sure.

july 12th

- We obtained the same results.

july 14th

- The plate is completely dry,the water evaporeted...WHY???? the first unsolved mystery of valencia iGEM 2008.

july 15th

- We prepared inoculum.

july 16th

- Trying to find out which is the best moment for activating UCP1...

july 17th

- Our lab electricity went of during the night. How many experiments will have ruined?

july 18th

- we carried out the pilot experiment. We see weird oscillations.

july 20th

- we stopped the shaking and carried out the experiment again to see whether the oscillations were related to the shaking.

july 21st

- We carried out the pilot experiment again. Weird results, we need to repeat it.

july 22nd

- We repaired our calorimeters. They were the reason for yesterday weird results.

july 23rd

- We repeated the pilot experiment.

july 24th

- Our male group members decided to carried out their own particular experiment involving parafilm, plasticine and teeth filling paste...

july 25th

- Another curious experiment was carried out: one thermocouple was covered with plasticine while the others weren't.

july 26th

- We obtain good results.

july 28th

microcalorimeter isn't OK, we try to repair and now.....calibrate once again!!!!

july 29th

why Saccharomyces cerevisiae don't want growth???? the temperature its OK like the medium.....PLEASE WAIT.......