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Team:Valencia/Project
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Project Overall
Stage 1
Uncoupling protein from the brown adipose tissue (UCP1) is a transporter that catalyzes a regulated discharged of the mitochondrial proton gradient. We aim to express this transmembrane protein in Saccaromyces Cerevisiae so as to be able to detect a temperature difference from the control.
We are using four different strains: one expresing UCP1, two mutants that overexpress UCP1, and a control one. All of this stains have been handed over by CSIC researcher Dr. Eduardo Rial.
To record temperature we are using calorimeters which consist on insulated flasks with attached thermocouples.
Stage 2
In a later stage, we expect to control this temperature differences to optimize the possible applications. Consequently, we will implement a regulatory system of UCP1 gene expression.
