Team:Virginia/Project

From 2008.igem.org

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<h3>BioPlastic</h3>
<h3>BioPlastic</h3>
<p>Making plastic a renewable resource.</p>
<p>Making plastic a renewable resource.</p>
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<p><i>Rastonia eutropha</i> naturally produces PHB for long-term carbon storage. Taking advantage of this microbes metabolic abilities, we've codon-optimized the 3 essential genes that code for the PHB biosynthesis pathway for expression in E. coli. The enzymes that are produced are PhaA, PhaB1 and PhaC1, which take acetyl-CoA to acetoacetyl-CoA, acetoacetyl-CoA to 3-hydroxybutyryl-CoA, and 3-hydroxybutyryl-CoA to poly-3-hydroxybutyrate (PHB), respectively. Why bother synthesizing PHB? It has the potential to replace polypropylene, a petroleum derivative, as main component of plastic materials.</p>
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Revision as of 03:17, 30 October 2008


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We'd like to thank our generous sponsors for making our work possible:
University of VirginiaduPont

Projects

Genetic Attenuator

Transcriptional attenuation is a very promising new tool for the specification and control of genetic transcription rates. The basic mechanism is simple: stop some fraction of the polymerases before they reach the desired gene to reduce transcription (and thereby translation) of the gene. Natural terminators already serve this role in their capacity to prevent transcription of undesired DNA, but a heretofore untapped resource is the potential inefficiency of this termination. For instance, imagine a terminator that interrupts only 50% of the polymerases transcribing it. If placed between two genes, this will result in 100% more copies of mRNA corresponding to the first gene than the second in the total transcript output. Without any sophisticated empirical testing and tuning (as is often required for promoter engineering), using a tool directly out of the BioBrick toolbox, an accurate and reliable ratio of gene transcription can been established.

 

Placeholder Sites

Cloning made easy!

 

BioPlastic

Making plastic a renewable resource.

Rastonia eutropha naturally produces PHB for long-term carbon storage. Taking advantage of this microbes metabolic abilities, we've codon-optimized the 3 essential genes that code for the PHB biosynthesis pathway for expression in E. coli. The enzymes that are produced are PhaA, PhaB1 and PhaC1, which take acetyl-CoA to acetoacetyl-CoA, acetoacetyl-CoA to 3-hydroxybutyryl-CoA, and 3-hydroxybutyryl-CoA to poly-3-hydroxybutyrate (PHB), respectively. Why bother synthesizing PHB? It has the potential to replace polypropylene, a petroleum derivative, as main component of plastic materials.

 

Adding to the Registry

More tools in the toolbox.