Team:Virginia/Protocols

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Hello World

Electrophoresis Gel

  • In a 100 mL Erlenmeyer flask, place and pour: 0.5 g Agar and 50 mL 1X TAE Buffer
  • Microwave for 30 seconds and then swirl the flask
  • Microwave for 15 seconds (caution: do not let the solution boil out of the flask)
  • Pipette 2 microliters of Ethidium Bromide into the gel solution and then swirl flask
    • Ethidium Bromide stains the plate for DNA to be observed under UV light
  • NOTE: Ethidium Bromide is a hazardous chemical and must be disposed of properly - this includes the gel itself
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