Team:Virginia/Results

From 2008.igem.org

(Difference between revisions)
Line 76: Line 76:
<h2>Results</h2>
<h2>Results</h2>
<img src="http://people.virginia.edu/~drt5p/VGEM/finalwiki/vgem-results.JPG">
<img src="http://people.virginia.edu/~drt5p/VGEM/finalwiki/vgem-results.JPG">
-
<p></p>
+
<p>The 2008 VGEM Team has had a very productive summer. We've designed a new technical standard, BioBrick Placeholders, that should help synthetic biologists with the assembly of composite BioBrick parts. We've synthesized inefficient terminators to serve as Genetic Attenuators, which should be a useful gene expression tool in that it can help control mRNA transcript levels. We've codon-optimized and expressed parts that code for an enzymatic pathway that produces polyhydroxybutyrate, a bioplastic. We've contributed a handful of other useful new parts including two new reporters, orange fluorescent protein (OFP) and strongly enhanced blue fluorescent protein (SBFP2), and a streptomycin resistance gene. We are currently working on incorporating our Genetic Attenuators in our PHB pathway to synthesize bioplastic.</p>
</div>
</div>

Revision as of 03:11, 30 October 2008


Home
People
Projects »
BioBricks
Results
Notebook
Locations of visitors to this page
We'd like to thank our generous sponsors for making our work possible:
University of VirginiaduPont

Results

The 2008 VGEM Team has had a very productive summer. We've designed a new technical standard, BioBrick Placeholders, that should help synthetic biologists with the assembly of composite BioBrick parts. We've synthesized inefficient terminators to serve as Genetic Attenuators, which should be a useful gene expression tool in that it can help control mRNA transcript levels. We've codon-optimized and expressed parts that code for an enzymatic pathway that produces polyhydroxybutyrate, a bioplastic. We've contributed a handful of other useful new parts including two new reporters, orange fluorescent protein (OFP) and strongly enhanced blue fluorescent protein (SBFP2), and a streptomycin resistance gene. We are currently working on incorporating our Genetic Attenuators in our PHB pathway to synthesize bioplastic.

Genetic Attenuator

A template plasmid that can be used to characterize terminators for use as Genetic Attenuators. Derived from Caitlin Conboy's work characterizing terminators in the registry.

 

BioPlastic

Making plastic a renewable resource.

SDS-PAGE result for enzymes in PHB synthesis pathway. From left to right: Marker, Control, PhaA, PhaB1, PhaC1

 

Adding to the Registry

More tools in the toolbox.

Orange Fluorescent Protein - OFP!

Retrieved from "http://2008.igem.org/Team:Virginia/Results"