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Team:Virginia/Results
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NotebookWe'd like to thank our generous sponsors for making our work possible:
Results
The 2008 VGEM Team has had a very productive summer. We've designed a new technical standard, BioBrick Placeholders, that should help synthetic biologists with the assembly of composite BioBrick parts. We've synthesized inefficient terminators to serve as Genetic Attenuators, which should be a useful gene expression tool in that it can help control mRNA transcript levels. We've codon-optimized and expressed parts that code for an enzymatic pathway that produces polyhydroxybutyrate, a bioplastic. We've contributed a handful of other useful new parts including two new reporters, orange fluorescent protein (OFP) and strongly enhanced blue fluorescent protein (SBFP2), and a streptomycin resistance gene. We are currently working on incorporating our Genetic Attenuators in our PHB pathway to synthesize bioplastic.
Genetic Attenuator
A template plasmid that can be used to characterize terminators for use as Genetic Attenuators. Derived from Caitlin Conboy's work characterizing terminators in the registry.
BioPlastic
Making plastic a renewable resource.
SDS-PAGE result for enzymes in PHB synthesis pathway. From left to right: Marker, Control, PhaA, PhaB1, PhaC1
Adding to the Registry
More tools in the toolbox.
Orange Fluorescent Protein - OFP!
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