Latest revision as of 21:27, 29 October 2008

A novel system for generation and screening for new interactors targeted against any bait

maximization of interaction between given bait and randomly generated protein by mutating sequence of the latter
interactor presentation on bacterial surface
selection of best bait interactor

Institutions whom we would like to thank for financial and technical support:

University of Warsaw	Faculty of Biology, University of Warsaw
The Universitatis Varsoviensis Foundation	Foundation for Polish Science

Warsaw University Foundation	Department of Molecular Biology, International Institute of Molecular and Cell Biology
Units belonging to the Faculty of Biology, University of Warsaw, who kindly provided access to laboratory space and equipment:
Department of Plant Molecular Biology	Department of Virology
Department of Bacterial Genetics	Institute of Genetics and Biotechnology

@@ Line 1: / Line 1: @@
 {{WarHead|headimg=2008.igem.org/wiki/images/f/fa/Logo_home.jpg}}
 <html>
-<br/>
-<br/>
+<h1>A novel system for generation and screening for new interactors targeted against any bait</h1>
-<div align="center"><img src="https://static.igem.org/mediawiki/2008/6/61/IGEMUWteam-small.gif"/></div>
+<p><ul>
-<p>Our team consists of 9 students of Faculty of Biology, University of
+<li>maximization of interaction between given bait and randomly generated protein by mutating sequence of the latter</li><li>
-Warsaw. This is the first time a group from Poland participates in the
+interactor presentation on bacterial surface</li><li>
-iGEM competition. Our plans were really amazing: we wanted to create
+selection of best bait interactor</li>
-system of biological machines which would allow to investigate protein
+</ul></p>
-interactions and simultaneously change sequence of one of them in
+<br>
-order to achieve the strongest possible interaction. To make it work
-we planned to express the tested protein in a bacterial strain in
+<div align="center"><img src="https://static.igem.org/mediawiki/2008/6/6a/Krag_zycia2.gif" usemap="#Map"/>
-which it would be mutated. Selection would occur outside outer
+<map name="Map" id="Map">
-bacterial membrane, where the protein would be attached. We planned
+  <area shape="poly" coords="596,397,458,396,454,334,598,331,593,337" href="https://2008.igem.org/Wiki/Team:Warsaw/igem_project.htm#selection" />
-that such selection system would allow us to search for antibodies
+<area shape="poly" coords="346,434,234,422,154,661,337,666" href="https://2008.igem.org/Wiki/Team:Warsaw/igem_project.htm#mutagenesis" />
-with new specificities or screen protein libraries. </p></td>
+<area shape="poly" coords="210,265,278,225,323,227,372,250,376,254,582,53,534,5,395,1,6,1,30,157" href="https://2008.igem.org/Wiki/Team:Warsaw/igem_project.htm#hunter" />
-   <td><img src="https://static.igem.org/mediawiki/2008/5/52/Spacer.gif" width="1" height="530" border="0" alt="" /></td>
+<area shape="poly" coords="185,349,211,388,23,470,9,385" href="https://2008.igem.org/Wiki/Team:Warsaw/igem_project.htm#selection" />
+<area shape="circle" coords="300,329,98" href="https://2008.igem.org/Wiki/Team:Warsaw/igem_project.htm" />
+</map>
+</div>
+</td>
+   <td></td>
    </tr>
 <tr><td>
-<h2>Institutions whom we would like to thank for financial and technical support:</h2>
+<h3>Institutions whom we would like to thank for financial and technical support:</h3>
 <table width="100%" border="0" cellspacing="10" cellpadding="0" align="center">
    <tr>
      <td align="center" valign="bottom"><a href="http://www.uw.edu.pl/en/"><img src="https://static.igem.org/mediawiki/2008/6/67/Uw.gif" width="100" height="99" border="0" /></a>
-     <a href="http://www.uw.edu.pl/en/"><h3>University of Warsaw</h3> </a></td>
+     <a href="http://www.uw.edu.pl/en/"><h4>University of Warsaw</h4> </a></td>
-     <td align="center" valign="bottom"><a href="http://www.fundacjauv.org.pl/"><img src="https://static.igem.org/mediawiki/2008/4/4d/Fundacja_uv-1.gif" width="160" height="65" border="0" /></a>
+     <td align="center" valign="bottom">
-     <a href="http://www.fundacjauv.org.pl/"><h3>The Universitatis Varsoviensis Fund </h3></a></td>
+<a href="http://www.biol.uw.edu.pl/_2008eng/"><img src="https://static.igem.org/mediawiki/2008/f/f3/Faculty-of-Biology.gif" width="57" height="75" border="0" /></a>
+     <a href="http://www.biol.uw.edu.pl/_2008eng/"><h4>Faculty of Biology, University of Warsaw </h4></a>
+</td>
    </tr>
    <tr>
-     <td align="center" valign="bottom"><a href="http://www.biol.uw.edu.pl/_2008eng/"><img src="https://static.igem.org/mediawiki/2008/f/f3/Faculty-of-Biology.gif" width="57" height="75" border="0" /></a>
+     <td align="center" valign="bottom"><a href="http://www.fundacjauv.org.pl/"><img src="https://static.igem.org/mediawiki/2008/4/4d/Fundacja_uv-1.gif" width="160" height="65" border="0" /></a>
-     <a href="http://www.biol.uw.edu.pl/_2008eng/"><h3>Faculty of Biology, University of Warsaw </h3></a>      </td>
+     <a href="http://www.fundacjauv.org.pl/"><h4>The Universitatis Varsoviensis Foundation </h4></a></td>
      <td align="center" valign="bottom"><a href="http://www.fnp.org.pl/ang/index.html"><img src="https://static.igem.org/mediawiki/2008/c/c9/LogoFNP1wers.gif" width="221" height="75" border="0" /></a>
-     <a href="http://www.fnp.org.pl/ang/index.html"><h3>Foundation for Polish Science </h3></a></td>
+     <a href="http://www.fnp.org.pl/ang/index.html"><h4>Foundation for Polish Science </h4></a></td>
    </tr>
    <tr>
@@ Line 37: / Line 44: @@
    </tr>
    <tr>
-     <td align="center" valign="bottom"><a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=529&amp;b_2501=5"><h3>Department of Bacterial Genetics </h3></a></td>
+    <td align="center" valign="bottom">
-     <td align="center" valign="bottom"><a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=526&amp;b_2501=5"><h3>Department of Virology</h3> </a></td>
+<a href="http://www.fuw.pl"><h4>Warsaw University Foundation</h4></a>
+</td>
+    <td align="center" valign="bottom">
+<a href="http://www.iimcb.gov.pl/zylicz_lab.php"><h4>Department of Molecular Biology, International Institute of Molecular and Cell Biology</h4></a>
+</td>
+  </tr>
+<tr>
+<td colspan="2" align="center" valign="bottom">
+<p>Units belonging to the <a href="http://www.biol.uw.edu.pl/_2008eng/">Faculty of Biology, University of Warsaw</a>, who kindly provided access to laboratory space and equipment:</p>
+</td>
+  <tr>
+     <td align="center" valign="bottom"><a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=541&amp;b_2501=5"><h4>Department of Plant Molecular Biology </h4></a></td>
+     <td align="center" valign="bottom"><a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=526&amp;b_2501=5"><h4>Department of Virology</h4> </a> </td>
    </tr>
    <tr>
-    <td align="center" valign="bottom"><a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=541&amp;b_2501=5"><h3>Department of Plant Molecular Biology </h3></a></td>
+     <td align="center" valign="bottom">
-    <td align="center" valign="bottom"><a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=534&amp;b_2501=5"><h3>Institute of Genetics and Biotechnology</h3></a> </td>
+<a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=529&amp;b_2501=5"><h4>Department of Bacterial Genetics </h4></a>
+</td>
+<td align="center" valign="bottom"><a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=534&amp;b_2501=5"><h4>Institute of Genetics and Biotechnology</h4></a></td>
    </tr>
 </table>