Team:Warsaw

From 2008.igem.org

(Difference between revisions)
 
(11 intermediate revisions not shown)
Line 1: Line 1:
{{WarHead|headimg=2008.igem.org/wiki/images/f/fa/Logo_home.jpg}}
{{WarHead|headimg=2008.igem.org/wiki/images/f/fa/Logo_home.jpg}}
<html>
<html>
 +
 +
<h1>A novel system for generation and screening for new interactors targeted against any bait</h1>
 +
<p><ul>
 +
<li>maximization of interaction between given bait and randomly generated protein by mutating sequence of the latter</li><li>
 +
interactor presentation on bacterial surface</li><li>
 +
selection of best bait interactor</li>
 +
</ul></p>
<br>
<br>
-
<p>We have developed a system allowing to search for antibodies with new specificities or screen protein libraries in order to generate interactors for a given bait. Our system changes a protein sequence to maximize its interaction with a given partner. Proteins with modified sequences are then directed to the bacterial outer membrane, where the best interactors are selected. Protein presented on the cell surface is fused with part of &#946;-lactamase protein, while its bait is fused with the complementing part of the enzyme and added to medium. The stronger the interaction between proteins of interest, the more efficient the binding of the two halves of &#946;-lactamase, leading to resistance to ampicillin and survival. Cells expressing less interacting variants die as they don't achieve sufficient complementation of the reporter enzyme. This allows us to select strains producing interactors for any given bait. </p>
 
-
<br>
 
-
<br>
 
-
 
-
Pisze tu bo boje się żejutro zapomnię - selekcja powinna być po mutagenezie, a nie przed, albo jeszcze lepiej po mutagenezie druga selekcja bo teraz to trochę wygląda jakbyśmy odrazu po mutagenezie otrzymywali białko oddziałujące
 
-
<br><br>
+
<div align="center"><img src="https://static.igem.org/mediawiki/2008/6/6a/Krag_zycia2.gif" usemap="#Map"/>
-
<div align="center"><img src="https://static.igem.org/mediawiki/2008/6/6a/Krag_zycia2.gif" usemap="#main_scheme"/>
+
<map name="Map" id="Map">
-
<map name="main_scheme" id="Map">
+
  <area shape="poly" coords="596,397,458,396,454,334,598,331,593,337" href="https://2008.igem.org/Wiki/Team:Warsaw/igem_project.htm#selection" />
-
<area shape="poly" coords="698,457,560,456,556,394,700,391,695,397" href="https://2008.igem.org/Wiki/Team:Warsaw/igem_project.htm#selection" />
+
<area shape="poly" coords="346,434,234,422,154,661,337,666" href="https://2008.igem.org/Wiki/Team:Warsaw/igem_project.htm#mutagenesis" />
-
<area shape="poly" coords="408,578,354,561,290,757,372,769" href="https://2008.igem.org/Wiki/Team:Warsaw/igem_project.htm#mutagenesis" />
+
<area shape="poly" coords="210,265,278,225,323,227,372,250,376,254,582,53,534,5,395,1,6,1,30,157" href="https://2008.igem.org/Wiki/Team:Warsaw/igem_project.htm#hunter" />
-
<area shape="poly" coords="246,319,323,267,378,266,413,281,437,295,624,63,537,6,398,2,9,2,33,158" href="https://2008.igem.org/Wiki/Team:Warsaw/igem_project.htm#hunter" />
+
<area shape="poly" coords="185,349,211,388,23,470,9,385" href="https://2008.igem.org/Wiki/Team:Warsaw/igem_project.htm#selection" />
 +
<area shape="circle" coords="300,329,98" href="https://2008.igem.org/Wiki/Team:Warsaw/igem_project.htm" />
 +
</map>
</div>
</div>
</td>
</td>
Line 19: Line 23:
   </tr>
   </tr>
<tr><td>
<tr><td>
-
<h2>Institutions whom we would like to thank for financial and technical support:</h2>
+
<h3>Institutions whom we would like to thank for financial and technical support:</h3>
<table width="100%" border="0" cellspacing="10" cellpadding="0" align="center">
<table width="100%" border="0" cellspacing="10" cellpadding="0" align="center">
   <tr>
   <tr>
     <td align="center" valign="bottom"><a href="http://www.uw.edu.pl/en/"><img src="https://static.igem.org/mediawiki/2008/6/67/Uw.gif" width="100" height="99" border="0" /></a>
     <td align="center" valign="bottom"><a href="http://www.uw.edu.pl/en/"><img src="https://static.igem.org/mediawiki/2008/6/67/Uw.gif" width="100" height="99" border="0" /></a>
-
     <a href="http://www.uw.edu.pl/en/"><h3>University of Warsaw</h3> </a></td>
+
     <a href="http://www.uw.edu.pl/en/"><h4>University of Warsaw</h4> </a></td>
     <td align="center" valign="bottom">
     <td align="center" valign="bottom">
<a href="http://www.biol.uw.edu.pl/_2008eng/"><img src="https://static.igem.org/mediawiki/2008/f/f3/Faculty-of-Biology.gif" width="57" height="75" border="0" /></a>
<a href="http://www.biol.uw.edu.pl/_2008eng/"><img src="https://static.igem.org/mediawiki/2008/f/f3/Faculty-of-Biology.gif" width="57" height="75" border="0" /></a>
-
     <a href="http://www.biol.uw.edu.pl/_2008eng/"><h3>Faculty of Biology, University of Warsaw </h3></a>       
+
     <a href="http://www.biol.uw.edu.pl/_2008eng/"><h4>Faculty of Biology, University of Warsaw </h4></a>       
</td>
</td>
   </tr>
   </tr>
   <tr>
   <tr>
     <td align="center" valign="bottom"><a href="http://www.fundacjauv.org.pl/"><img src="https://static.igem.org/mediawiki/2008/4/4d/Fundacja_uv-1.gif" width="160" height="65" border="0" /></a>
     <td align="center" valign="bottom"><a href="http://www.fundacjauv.org.pl/"><img src="https://static.igem.org/mediawiki/2008/4/4d/Fundacja_uv-1.gif" width="160" height="65" border="0" /></a>
-
     <a href="http://www.fundacjauv.org.pl/"><h3>The Universitatis Varsoviensis Fund </h3></a></td>
+
     <a href="http://www.fundacjauv.org.pl/"><h4>The Universitatis Varsoviensis Foundation </h4></a></td>
     <td align="center" valign="bottom"><a href="http://www.fnp.org.pl/ang/index.html"><img src="https://static.igem.org/mediawiki/2008/c/c9/LogoFNP1wers.gif" width="221" height="75" border="0" /></a>
     <td align="center" valign="bottom"><a href="http://www.fnp.org.pl/ang/index.html"><img src="https://static.igem.org/mediawiki/2008/c/c9/LogoFNP1wers.gif" width="221" height="75" border="0" /></a>
-
     <a href="http://www.fnp.org.pl/ang/index.html"><h3>Foundation for Polish Science </h3></a></td>
+
     <a href="http://www.fnp.org.pl/ang/index.html"><h4>Foundation for Polish Science </h4></a></td>
   </tr>
   </tr>
   <tr>
   <tr>
Line 41: Line 45:
   <tr>
   <tr>
     <td align="center" valign="bottom">
     <td align="center" valign="bottom">
-
<a href="http://www.fuw.pl"><H3>Warsaw University Foundation</h3></a>
+
<a href="http://www.fuw.pl"><h4>Warsaw University Foundation</h4></a>
</td>
</td>
     <td align="center" valign="bottom">
     <td align="center" valign="bottom">
-
<a href="http://www.iimcb.gov.pl/zylicz_lab.php"><h3>Department of Molecular Biology, IIMCB</h3></a>
+
<a href="http://www.iimcb.gov.pl/zylicz_lab.php"><h4>Department of Molecular Biology, International Institute of Molecular and Cell Biology</h4></a>
</td>
</td>
   </tr>
   </tr>
Line 51: Line 55:
<tr>
<tr>
<td colspan="2" align="center" valign="bottom">
<td colspan="2" align="center" valign="bottom">
-
<p>Departments belonging to the <a href="http://www.biol.uw.edu.pl/_2008eng/">Faculty of Biology, University of Warsaw</a>, who kindly provided access to laboratory space and equipment:</p>
+
<p>Units belonging to the <a href="http://www.biol.uw.edu.pl/_2008eng/">Faculty of Biology, University of Warsaw</a>, who kindly provided access to laboratory space and equipment:</p>
</td>
</td>
   <tr>
   <tr>
-
     <td align="center" valign="bottom"><a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=541&amp;b_2501=5"><h3>Department of Plant Molecular Biology </h3></a></td>
+
     <td align="center" valign="bottom"><a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=541&amp;b_2501=5"><h4>Department of Plant Molecular Biology </h4></a></td>
-
     <td align="center" valign="bottom"><a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=526&amp;b_2501=5"><h3>Department of Virology</h3> </a> </td>
+
     <td align="center" valign="bottom"><a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=526&amp;b_2501=5"><h4>Department of Virology</h4> </a> </td>
   </tr>
   </tr>
   <tr>
   <tr>
     <td align="center" valign="bottom">
     <td align="center" valign="bottom">
-
<a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=529&amp;b_2501=5"><h3>Department of Bacterial Genetics </h3></a>
+
<a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=529&amp;b_2501=5"><h4>Department of Bacterial Genetics </h4></a>
</td>
</td>
-
<td align="center" valign="bottom"><a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=534&amp;b_2501=5"><h3>Institute of Genetics and Biotechnology</h3></a></td>
+
<td align="center" valign="bottom"><a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=534&amp;b_2501=5"><h4>Institute of Genetics and Biotechnology</h4></a></td>
   </tr>
   </tr>
</table>
</table>

Latest revision as of 21:27, 29 October 2008

Gallery Bricks Notebook Team Project Home

A novel system for generation and screening for new interactors targeted against any bait

  • maximization of interaction between given bait and randomly generated protein by mutating sequence of the latter
  • interactor presentation on bacterial surface
  • selection of best bait interactor


Institutions whom we would like to thank for financial and technical support:

University of Warsaw

Faculty of Biology, University of Warsaw

The Universitatis Varsoviensis Foundation

Foundation for Polish Science

Warsaw University Foundation

Department of Molecular Biology, International Institute of Molecular and Cell Biology

Units belonging to the Faculty of Biology, University of Warsaw, who kindly provided access to laboratory space and equipment:

Department of Plant Molecular Biology

Department of Virology

Department of Bacterial Genetics

Institute of Genetics and Biotechnology