Team:Warsaw

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We want to note that our initial plans were very different from what we actually managed to do. Our plans were really amazing: we wanted to create system of biological machines which would allow to investigate protein interactions and simultaneously change sequence of one of them in order to achieve the strongest possible interaction. To make it work we planned to put the DNA sequence of tested protein on low-copy plasmid and transform it to bacterial strain in which it would be mutated (lets call the strain ‘slot machine’). Parts present on this plasmid would cause the protein to be attached to outer bacterial membrane, where the selection would occur. We planned that such selection system would allow us to search for antibodies with new specificities or screen protein libraries.

Institutions whom we would like to thank for financial and technical support:

University of Warsaw

The Universitatis Varsoviensis Fund

Faculty of Biology, University of Warsaw

Foundation for Polish Science

Department of Bacterial Genetics

Department of Virology

Department of Plant Molecular Biology

Institute of Genetics and Biotechnology