Team:Warsaw/Calendar-Main/10 June 2008

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Michał L., Ewa, Marcin

  • Plasmid isolation from overnight culture of our transformants.
  • Control digest of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and SalI.
  • Control digest of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and EcoRI.
  • Control digest of pMPM-T5 + T7 RNA-polymerase with EcoRI.
  • Control digest of pMPM-T5 + T7 RNA-polymerase with BamHI and XhoI.
  • DNA gel electrophoresis of digestion products.
  • Picking out proper colonies of transformants.