Visit in US Embassy

Visas have been accorded to the whole team.

Preparation of BioBricks

Michał K.

1. Digest of RFP(?????)+deltaA with EcoRI and SacI (BamHI buffer), dephosphorylation with CIAP
2. Gel electrophoresis and gel-out of proper band 2200 bp.
3. Ligation of digested 5kb (??????) vector from (30 September) with pT7_omega_link and RFP(?????) with pT7_alpha fragment (1 hr).

Piotr

1. Transformation of TOP10 with above ligations.
2. Tranformants planting on LB with kanamycin.