Team:Warsaw/Calendar-Main/10 October 2008
From 2008.igem.org
(Difference between revisions)
MKrzyszton (Talk | contribs) |
MKrzyszton (Talk | contribs) |
||
Line 7: | Line 7: | ||
<h3>Preparation of pT7_alpha_link</h3> | <h3>Preparation of pT7_alpha_link</h3> | ||
- | <h4>Michał K.</h4> | + | <h4>Michał K., Piotr</h4> |
<ol> | <ol> | ||
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_purification_after_enzymatic_reaction">Clean-up</a> of overnight digest reaction. </li> | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_purification_after_enzymatic_reaction">Clean-up</a> of overnight digest reaction. </li> | ||
Line 14: | Line 14: | ||
<li>Gel electrophoresis and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper band - 2200 bp. </li> | <li>Gel electrophoresis and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper band - 2200 bp. </li> | ||
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of digested <A href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a> carrying <a href=http://partsregistry.org/wiki/index.php?title=Part:BBa_K103003>ΔA (BBa_K103003)</a> with pT7_alpha fragment (1 hr).</li> | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of digested <A href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a> carrying <a href=http://partsregistry.org/wiki/index.php?title=Part:BBa_K103003>ΔA (BBa_K103003)</a> with pT7_alpha fragment (1 hr).</li> | ||
+ | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> with above ligation.</li> | ||
+ | <li> Tranformants planting on LB with ampicillin. </li> | ||
+ | </ol> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
Revision as of 09:02, 27 October 2008
Visit in US EmbassyVisas have been accorded to the whole team. Preparation of pT7_alpha_linkMichał K., Piotr
Preparation of BioBricksMichał K.
Piotr
Michał K.
|