Team:Warsaw/Calendar-Main/10 September 2008

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<h3>Tests for ampicillin resistance given by protein added to medium</h3><h4>Piotr</h4>
<h3>Tests for ampicillin resistance given by protein added to medium</h3><h4>Piotr</h4>
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<ol><li>Inoculation of all OmpA variants of constructs to LB + 0.25 mM IPTG, kanamycin, ampicillin (50 μg/ml) and 100 μg of protein (A-alpha, Z-alpha and Z-omega; negative control without protein).</li>
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<ol><li>Inoculation of all OmpA_variants from previous day to LB + 0.25 mM IPTG, kanamycin, ampicillin (50 μg/ml) and various cocnentration of protein (A-alpha, Z-alpha and Z-omega; negative control without protein).</li>
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<li>Measurement of growth after 5 hours and on next day.</li></ol>
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<li>Measurement of growth after 6 hours.</li></ol>
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Tabela z wynikami z exela- minimalna ilość biąłka do porzywki (ilość bilałka dodaną do porztwki przeliczono na nanomole białka- tu dać ile lażde z białek ma masy molowej):
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Tests for ampicillin resistance given by protein added to medium

Piotr

  1. Inoculation of all OmpA_variants from previous day to LB + 0.25 mM IPTG, kanamycin, ampicillin (50 μg/ml) and various cocnentration of protein (A-alpha, Z-alpha and Z-omega; negative control without protein).
  2. Measurement of growth after 6 hours.
Tabela z wynikami z exela- minimalna ilość biąłka do porzywki (ilość bilałka dodaną do porztwki przeliczono na nanomole białka- tu dać ile lażde z białek ma masy molowej):

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