Team:Warsaw/Calendar-Main/11 July 2008
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(Difference between revisions)
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<p>'''Preparation of construct A-omega'''<br> | <p>'''Preparation of construct A-omega'''<br> | ||
- | 1. PCR | + | 1. PCR A<br> |
+ | template DNA - pKS-A4 1ul | ||
+ | primer APNot - 2 ul | ||
+ | primer ALhomo2 - 2 ul | ||
+ | Pfu polymerase buffer + Mg2+ (from Fermentas) - 5 ul | ||
+ | dNTPs - 1 ul | ||
+ | Pfu turbo - 0.5 ul | ||
+ | H2o - 38.5 ul | ||
+ | |||
+ | program: | ||
+ | 1. 95oC 3' | ||
+ | 2. 95oC 30" | ||
+ | 3.62oC 45" | ||
+ | 4.72oC 45" | ||
+ | 5.72oC 10' | ||
+ | 6. keeping in 4oC | ||
</p> | </p> | ||
Revision as of 15:48, 26 September 2008