Team:Warsaw/Calendar-Main/11 July 2008
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<h3>Cloning of protein Z DNA to OmpA constructs</h3> | <h3>Cloning of protein Z DNA to OmpA constructs</h3> | ||
<p> 2 colonies was inoculated to liquid LB broth with kanamycin</p> | <p> 2 colonies was inoculated to liquid LB broth with kanamycin</p> | ||
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+ | <h3>Preparation of construct pKS with A protein</h3> | ||
+ | <p><ol> | ||
+ | <li> Colony PCR on colonies from plates with transformations pKS-A <br> | ||
+ | Primers used: | ||
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+ | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a> | ||
+ | </li> | ||
+ | <li> Confirmed transformant colonies inoculated to liquid LB with ampicillin</li></ol></p> | ||
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Revision as of 14:38, 11 October 2008
Preparation of constructs with OmpA protein fusions
Cloning of protein Z DNA to OmpA constructs2 colonies was inoculated to liquid LB broth with kanamycin Preparation of construct pKS with A protein
Cloning omega-A fusion on pKS (second attempt)Michał L., Ewa, Marcin:We had to start form scratch with this one.
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