"
Team:Warsaw/Calendar-Main/11 July 2008
From 2008.igem.org
(Difference between revisions)
MKrzyszton (Talk | contribs) |
MKrzyszton (Talk | contribs) |
||
| Line 7: | Line 7: | ||
<li> Confirmed transformant colonies inoculated to liquid LB with kanamycin.</li> | <li> Confirmed transformant colonies inoculated to liquid LB with kanamycin.</li> | ||
</ol></p> | </ol></p> | ||
| + | |||
| + | <h3>Preparation of construct pKS with A protein</h3> | ||
| + | <p><ol> | ||
| + | <li> Colony PCR on colonies from plates with transformations pKS-A <br> | ||
| + | Primers used: | ||
| + | |||
| + | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a> | ||
| + | </li> | ||
| + | <li> Confirmed transformant colonies inoculated to liquid LB with ampicillin</li></ol></p> | ||
| + | |||
| + | |||
Revision as of 15:09, 11 October 2008
 |
|
||||||||
 |
|
||||||||
Preparation of constructs with OmpA protein fusions
Preparation of construct pKS with A protein
|
|
