Team:Warsaw/Calendar-Main/11 July 2008

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<li> Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on colonies from plates with transformations OmpA_alpha. </li>
<li> Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on colonies from plates with transformations OmpA_alpha. </li>
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<li> Gel electrophoresis.</li>
<li> Confirmed transformant colonies inoculated to liquid LB with kanamycin.</li>
<li> Confirmed transformant colonies inoculated to liquid LB with kanamycin.</li>
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</ol></p>

Revision as of 22:36, 11 October 2008

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Preparation of constructs with OmpA protein fusions

Michał K.

  1. Colony PCR on colonies from plates with transformations OmpA_alpha.
  2. Gel electrophoresis.
  3. Confirmed transformant colonies inoculated to liquid LB with kanamycin.

Preparation of construct pKS with A protein

Michał L., Ewa, Marcin

  1. Colony PCR on colonies from plates with transformations pKS-A.
    Primers used: AL+SacI and AP+NotI
  2. Confirmed transformant colonies inoculated to liquid LB with ampicillin.

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