Team:Warsaw/Calendar-Main/11 July 2008

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<h3>Preparation of construct pKS with A protein</h3>
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<h3>Preparation of construct <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pKSII+A>pKS with A protein</a></h3>
<h4>Michał L., Ewa, Marcin</h4>
<h4>Michał L., Ewa, Marcin</h4>
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<li> Colony PCR on colonies from plates with transformations pKS-A. <br>
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<li> Colony PCR on colonies from plates with transformations <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pKSII+A>pKS-A</a>. <br>
Primers used:
Primers used:

Revision as of 11:48, 13 October 2008

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Preparation of constructs with OmpA protein fusions

Michał K.

  1. Colony PCR on colonies from plates with transformations OmpA_alpha.
  2. Gel electrophoresis.
  3. Confirmed transformant colonies inoculated to liquid LB with kanamycin.

Preparation of construct pKS with A protein

Michał L., Ewa, Marcin

  1. Colony PCR on colonies from plates with transformations pKS-A.
    Primers used: AL+SacI and AP+NotI
  2. Gel electrophoresis.
  3. Confirmed transformant colonies inoculated to liquid LB with ampicillin.

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