Team:Warsaw/Calendar-Main/11 July 2008
From 2008.igem.org
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- | + | <img src="https://static.igem.org/mediawiki/2008/9/9c/11_th_july.jpg" width=300 /><var>Fig. 1. Control BamHI and NotI digests of plasmids, which were used in successful colony PCRs <b></b><br> | |
+ | 1. Marker<br> | ||
+ | 2. slot 1. from yesterday's Omp_A_alpha colony PCR<br> | ||
+ | 2. slot 14. from yesterday's Omp_A_alpha colony PCR<br> | ||
+ | 2. slot 4. from yesterday's Omp_A_omega colony PCR<br> | ||
+ | 2. slot 10. from yesterday's Omp_A_omega colony PCR<br> | ||
Revision as of 15:14, 25 October 2008
Preparation of constructs with OmpA protein fusionsMichał K.
Preparation of construct pKS with A proteinMichał L., Ewa, Marcin
Cloning of protein Z DNA to pET15b-OmpA-alpha in place of OmpAPiotr, AntoniIsolation of plasmids from cultures inoculated on previous day. Fig. 1. Control BamHI and NotI digests of plasmids, which were used in successful colony PCRs1. Marker 2. slot 1. from yesterday's Omp_A_alpha colony PCR 2. slot 14. from yesterday's Omp_A_alpha colony PCR 2. slot 4. from yesterday's Omp_A_omega colony PCR 2. slot 10. from yesterday's Omp_A_omega colony PCR |