Team:Warsaw/Calendar-Main/11 July 2008
From 2008.igem.org
(Difference between revisions)
MKrzyszton (Talk | contribs) |
|||
Line 26: | Line 26: | ||
primers on colonies from plates with transformations OmpA_alpha (annealing temperature - 55°C,45 s of elongation step). </li> | primers on colonies from plates with transformations OmpA_alpha (annealing temperature - 55°C,45 s of elongation step). </li> | ||
- | <li> Gel electrophoresis.</li> | + | <li> Gel electrophoresis (Fig. 1.).</li> |
<li> Confirmed transformant colonies inoculated to liquid LB with kanamycin.</li> | <li> Confirmed transformant colonies inoculated to liquid LB with kanamycin.</li> | ||
</ol></p> | </ol></p> |
Revision as of 16:51, 26 October 2008
Preparation of construct pKS with A proteinMichał L., Ewa, Marcin
Cloning of protein Z DNA to pET15b-OmpA-alpha in place of OmpAPiotr, AntoniIsolation of plasmids from cultures inoculated on previous day. Preparation of constructs: OmpA_alpha and OmpA_omega #2Michał K.
Upper 1. Marker 2-13. colony PCR products carrying probable OmpA-alpha Lower 1. Marker 2-13. colony PCR products carrying probable OmpA-alpha |