Team:Warsaw/Calendar-Main/11 September 2008

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Inoculation of all OmpA variants of constructs and overnight induction.<br>
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<p><ol><li>Inoculation of pACYC177+OmpA_variants from previous day to LB + 0.25 mM IPTG, kanamycin and 50 μl/ml ampicillin with minimum concentration of prey (yesterday's results).</li>
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<li>Measurement of growth after 6 hours - 2-fold growth increase in presence of interacting proteins (<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-omega>pACYC177+OmpA_A_omega</a> twice as much as <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega>pACYC177+OmpA_omega</a> with protein Z_alpha, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-omega>pACYC177+OmpA_Z_omega</a> twice as much as
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<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega>pACYC177+OmpA_omega </a>with protein A_alpha and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-alpha>pACYC177+OmpA_Z_alpha</a> twice as much as <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-alpha>pACYC177+OmpA_alpha</a> with protein Z_omega.)!!!!</li></ol></p>
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<p>We're fighting hard to obtain funds for the visit on iGEM2008 Jamboree!</p><br><br>
 
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<h3>Repetition of test for ampicillin resistance given by protein added to medium</h3><h4>Michał L., Ewa, Marcin</h4>
 
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<ol><li>We follow the <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#Testing%20various%20hunter/prey%20combinations">protocol</a>.
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<ul><li>Inoculation of <i>E. coli</i> TOP10 carrying OmpA variants into liquid LB with kanamycin and IPTG.</li></ul></li></ol>
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<h3>We're fighting hard to obtain funds for the visit on iGEM2008 Jamboree!</h3>
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<h4></h4>
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Tests for ampicillin resistance given by protein added to medium

Piotr

  1. Inoculation of pACYC177+OmpA_variants from previous day to LB + 0.25 mM IPTG, kanamycin and 50 μl/ml ampicillin with minimum concentration of prey (yesterday's results).
  2. Measurement of growth after 6 hours - 2-fold growth increase in presence of interacting proteins (pACYC177+OmpA_A_omega twice as much as pACYC177+OmpA_omega with protein Z_alpha, pACYC177+OmpA_Z_omega twice as much as pACYC177+OmpA_omega with protein A_alpha and pACYC177+OmpA_Z_alpha twice as much as pACYC177+OmpA_alpha with protein Z_omega.)!!!!

We're fighting hard to obtain funds for the visit on iGEM2008 Jamboree!

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