Team:Warsaw/Calendar-Main/12 July 2008
From 2008.igem.org
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- | <h3>Preparation of constructs with OmpA protein fusions | + | <h3>Preparation of constructs with OmpA protein fusions and Cloning of protein Z DNA to OmpA constructs</h3> |
<p><ol> | <p><ol> | ||
- | <li> Isolation of plasmids from cultures inocluated on previous day (pCACYC177 + OmpA_alpha and pACYC177+OmpA_Z_omega). </li> | + | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation of plasmids</a> from cultures inocluated on previous day (pCACYC177 + OmpA_alpha and pACYC177+OmpA_Z_omega). </li> |
- | <li> Control | + | <li> Control |
+ | <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of isolated plasmids with BamHI and SacI (we found good clones for both ligations).</li> | ||
</ol></p> | </ol></p> | ||
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<li>primer | <li>primer | ||
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI_N">AP+NotI_N</a> - 2 µl</li> | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI_N">AP+NotI_N</a> - 2 µl</li> | ||
- | <li>Pfu buffer with | + | <li>Pfu buffer with Mg<sup>2+</sup> - 5 µl</li> |
<li>dNTPs - 1 µl</li> | <li>dNTPs - 1 µl</li> | ||
- | <li> | + | <li>H<sub>2</sub>o - 22 µl</li> |
<li>Program:</li> | <li>Program:</li> | ||
<ol> | <ol> |
Revision as of 17:56, 1 October 2008
Preparation of constructs with OmpA protein fusions and Cloning of protein Z DNA to OmpA constructs
Linked PCR Omega-A
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