Team:Warsaw/Calendar-Main/12 July 2008

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<h3>Preparation of constructs with OmpA protein fusions</h3> <h4>Michał K.</h4>
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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation of plasmids</a> from cultures inocluated on previous day (pCACYC177 + OmpA_alpha). </li>
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<li> Control
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of isolated plasmids with BamHI and SacI (BamHI buffer) - we found good clones.</li>
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Revision as of 15:19, 11 October 2008

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Preparation of constructs with OmpA protein fusions

Michał K.

  1. Isolation of plasmids from cultures inocluated on previous day (pCACYC177 + OmpA_alpha).
  2. Control digest of isolated plasmids with BamHI and SacI (BamHI buffer) - we found good clones.