Team:Warsaw/Calendar-Main/12 July 2008
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<h3>Preparation of construct <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pKSII+A>pKS with A protein</a></h3> <h4>Michał L., Ewa, Marcin</h4> | <h3>Preparation of construct <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pKSII+A>pKS with A protein</a></h3> <h4>Michał L., Ewa, Marcin</h4> | ||
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<li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with NdeI and NotI. Two positives obtained (proper band: ~200 bp visible).</li> | <li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with NdeI and NotI. Two positives obtained (proper band: ~200 bp visible).</li> | ||
<li>One of positive plasmids transformed into <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> and plated on LB+amp, overnight, for further isolation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BZ-alpha>pET15b+Z+alpha</a> vector.</li> | <li>One of positive plasmids transformed into <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> and plated on LB+amp, overnight, for further isolation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BZ-alpha>pET15b+Z+alpha</a> vector.</li> | ||
+ | </ol></p> | ||
+ | <h3>Preparation of constructs: OmpA_alpha and OmpA_omega #2</h3> <h4>Michał K.</h4> | ||
+ | <p><ol> | ||
+ | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation of plasmids</a> from cultures inocluated on previous day (<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-alpha>pCACYC177 + OmpA_alpha</a>). </li> | ||
+ | <li> Control | ||
+ | <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of isolated plasmids with BamHI and SacI (BamHI buffer).</li> <li> Gel electrophoresis - we found good clones.</li> | ||
</ol></p> | </ol></p> | ||
Revision as of 16:45, 26 October 2008
Preparation of construct pKS with A proteinMichał L., Ewa, Marcin
Cloning of protein Z DNA to pET15b-OmpA-alpha in place of OmpAPiotr, Antoni
Preparation of constructs: OmpA_alpha and OmpA_omega #2Michał K.
1. Marker 2-4. digested plasmids which had a colony PCR product |