Team:Warsaw/Calendar-Main/12 July 2008

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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation of plasmid DNA</a> from cultures inocluated on previous day. </li>
<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation of plasmid DNA</a> from cultures inocluated on previous day. </li>
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<li> Control <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of isolated plasmid with SacI and NotI; 2 h</li>
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<li> Control <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of isolated plasmid with SacI and NotI (BamHI buffer) - 2 h.</li>
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<li> Gel electrophoresis of digested DNA</li>
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<li> Gel electrophoresis of digested DNA.</li>
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<li> We are glad to find the proper clone ;-) and inoculate liquid LB with ampicillin to freeze bacteria carrying pKS-A construct</li></ol>
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<li> We are glad to find the proper clone and inoculate liquid LB with ampicillin to freeze bacteria carrying pKS-A construct.</li></ol>
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Revision as of 15:22, 11 October 2008

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Preparation of constructs with OmpA protein fusions

Michał K.

  1. Isolation of plasmids from cultures inocluated on previous day (pCACYC177 + OmpA_alpha).
  2. Control digest of isolated plasmids with BamHI and SacI (BamHI buffer) - we found good clones.

Preparation of construct pKS with A protein

Michał L., Ewa, Marcin

  1. Isolation of plasmid DNA from cultures inocluated on previous day.
  2. Control digest of isolated plasmid with SacI and NotI (BamHI buffer) - 2 h.
  3. Gel electrophoresis of digested DNA.
  4. We are glad to find the proper clone and inoculate liquid LB with ampicillin to freeze bacteria carrying pKS-A construct.

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