Preparation of constructs with OmpA protein fusions

Michał K.

1. Isolation of plasmids from cultures inocluated on previous day (pCACYC177 + OmpA_alpha).
2. Control digest of isolated plasmids with BamHI and SacI (BamHI buffer) - we found good clones.

Preparation of construct pKS with A protein

1. Isolation of plasmid DNA from cultures inocluated on previous day.
2. Control digest of isolated plasmid with SacI and NotI; 2 h
3. Gel electrophoresis of digested DNA
4. We are glad to find the proper clone ;-) and inoculate liquid LB with ampicillin to freeze bacteria carrying pKS-A construct