Preparation of constructs with OmpA protein fusions

Michał K.

1. Isolation of plasmids from cultures inocluated on previous day (pCACYC177 + OmpA_alpha).
2. Control digest of isolated plasmids with BamHI and SacI (BamHI buffer).
3. Gel electrophoresis- we found good clones.

Preparation of construct pKS with A protein

Michał L., Ewa, Marcin

1. Isolation of plasmid DNA from cultures inocluated on previous day.
2. Control digest of isolated plasmid with SacI and NotI (BamHI buffer) - 2 h.
3. Gel electrophoresis of digested DNA.
4. We are glad to find the proper clone and inoculate liquid LB with ampicillin to freeze bacteria carrying pKS-A construct.