Team:Warsaw/Calendar-Main/12 May 2008

From 2008.igem.org

Revision as of 21:10, 1 October 2008 by MKrzyszton (Talk | contribs)

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
next notebook entry

 


Michał K.:

  1. Restriction digest of pTrc99A-AID with HindIII and NcoI (1x Tango buffer).
  2. DNA electrophoresis and isolation of DNA encoding AID from proper band (600 bp).
  3. Restriction digest of pMPM-T5 with HindIII and NcoI (1x Tango buffer), dephosphorylation (CIAP).
  4. DNA electrophoresis and isolation of pMPM-T5 vector from proper band (4250 bp).
  5. Electrophoresis of isolated DNA
  6. Ligation of isolated DNA fragments.