Team:Warsaw/Calendar-Main/13 June 2008
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<p>Each reaction was carried out with 15 and 20 cycles and in temperature gradient from 55 to 75 degrees.</p> | <p>Each reaction was carried out with 15 and 20 cycles and in temperature gradient from 55 to 75 degrees.</p> | ||
- | <img src="https://static.igem.org/mediawiki/2008/2/2b/PCR_Ompa_Alpha_Omega_WAW1.jpg" width=400/> | + | <img src="https://static.igem.org/mediawiki/2008/2/2b/PCR_Ompa_Alpha_Omega_WAW1.jpg" width=400/><br> |
- | < | + | |
<img src="https://static.igem.org/mediawiki/2008/8/82/PCR_Ompa_Alpha_Omega_WAW2.jpg" width=400/><br> | <img src="https://static.igem.org/mediawiki/2008/8/82/PCR_Ompa_Alpha_Omega_WAW2.jpg" width=400/><br> | ||
<img src="https://static.igem.org/mediawiki/2008/7/76/PCR_Ompa_Alpha_Omega_WAW3.jpg" width=400/><br> | <img src="https://static.igem.org/mediawiki/2008/7/76/PCR_Ompa_Alpha_Omega_WAW3.jpg" width=400/><br> | ||
<img src="https://static.igem.org/mediawiki/2008/6/68/PCR_Ompa_Alpha_Omega_WAW4.jpg" width=200/> | <img src="https://static.igem.org/mediawiki/2008/6/68/PCR_Ompa_Alpha_Omega_WAW4.jpg" width=200/> | ||
+ | <var>Gradient PCR products: 1, 14 and 27-DNA ladder; <br> | ||
+ | 2 to 8 - gradient PCR for Alpha carried out with 15 cycles; <br> | ||
+ | 9 to 16 - gradient PCR for Alpha carried out with 20 cycles; <br> | ||
+ | 17 to 23 - gradient PCR for Omega carried out with 15 cycles; <br> | ||
+ | 24 to 31 - gradient PCR for Omega carried out with 20 cycles; <br> | ||
+ | 32 to 38 - gradient PCR for OmpA carried out with 15 cycles; <br> | ||
+ | 39 to 46 - gradient PCR for OmpA carried out with 20 cycles. <br> | ||
+ | Product od each variant of PCR is in 7 lanes: 1<sup>st</sup> is the lower border annealing temperature (55 degrees), 6<sup>th</sup> is the higher annealing temperature (75 degrees) and 7<sup>th</sup> is negative control without template DNA.</var> | ||
</html> | </html> | ||
Revision as of 00:19, 12 October 2008
Optimisation of primers from 10.06Michał K.
Each reaction was carried out with 15 and 20 cycles and in temperature gradient from 55 to 75 degrees. Gradient PCR products: 1, 14 and 27-DNA ladder; 2 to 8 - gradient PCR for Alpha carried out with 15 cycles; 9 to 16 - gradient PCR for Alpha carried out with 20 cycles; 17 to 23 - gradient PCR for Omega carried out with 15 cycles; 24 to 31 - gradient PCR for Omega carried out with 20 cycles; 32 to 38 - gradient PCR for OmpA carried out with 15 cycles; 39 to 46 - gradient PCR for OmpA carried out with 20 cycles. Product od each variant of PCR is in 7 lanes: 1st is the lower border annealing temperature (55 degrees), 6th is the higher annealing temperature (75 degrees) and 7th is negative control without template DNA.
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