Team:Warsaw/Calendar-Main/13 September 2008

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<h3>Repetition of test for ampicillin resistance given by protein added to medium</h3><h4>Michał L., Ewa, Marcin</h4>
<h3>Repetition of test for ampicillin resistance given by protein added to medium</h3><h4>Michał L., Ewa, Marcin</h4>
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<p>Results of the test:</p>
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<p>Results of the test:</p><br>
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<table id="result">
<table id="result">
<tr><th>OmpA variant ("hunter")</th><th>"Prey" variant</th><th>Growth with prey</th><th>Growth without prey</th></tr>
<tr><th>OmpA variant ("hunter")</th><th>"Prey" variant</th><th>Growth with prey</th><th>Growth without prey</th></tr>
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<tr><th>Ompa-A-omega</th><td>His+Z+alpha</td><td>yes</td><td>no</td></tr>
<tr><th>Ompa-A-omega</th><td>His+Z+alpha</td><td>yes</td><td>no</td></tr>
<tr><th>OmpA-omega</th><td>His+A+alpha</td><td>no</th><td>no</td></tr>
<tr><th>OmpA-omega</th><td>His+A+alpha</td><td>no</th><td>no</td></tr>
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<tr><th>Ompa-Z-alpha</th><td>His+A+omega??</td><td>yes</td><td>no</td></tr>
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<tr><th>Ompa-Z-alpha</th><td>His+A+omega</td><td>yes</td><td>no</td></tr>
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<tr><th>Ompa-Z-alpha</th><td>His+A+omega??</td><td>yes</td><td>no</td></tr>
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<tr><th>Ompa-Z-alpha</th><td>His+A+omega</td><td>yes</td><td>no</td></tr>
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</table>
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</table><br>
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/*
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<br>
<p>Conclusions:  
<p>Conclusions:  
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</p>
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<ol>
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<li>None of tested constructs give resistance to ampicillin without prey protein added to culture medium.</li>
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<li>Interaction between hunter and prey proteins connected to beta-lactamase makes cells ampicillin resistant</li>
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</ol>
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</p>
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*/
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Repetition of test for ampicillin resistance given by protein added to medium

Michał L., Ewa, Marcin

Results of the test:


OmpA variant ("hunter")"Prey" variantGrowth with preyGrowth without prey
OmpA-alphaHis+Z+omeganono
OmpA-alphaHis+Z+omeganono
OmpA-A-alphaHis+Z+omegayesno
OmpA-Z-omegaHis+A+alphayesno
OmpA-omega-A-alphaHis+Z+omegayesno
Ompa-A-omegaHis+Z+alphayesno
Ompa-A-omegaHis+Z+alphayesno
OmpA-omegaHis+A+alphanono
Ompa-Z-alphaHis+A+omegayesno
Ompa-Z-alphaHis+A+omegayesno
/*

Conclusions:

  1. None of tested constructs give resistance to ampicillin without prey protein added to culture medium.
  2. Interaction between hunter and prey proteins connected to beta-lactamase makes cells ampicillin resistant

*/