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Team:Warsaw/Calendar-Main/14 July 2008
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| - | <h3>Cloning of protein Z DNA to OmpA constructs</h3> | + | <h3>Cloning of protein Z DNA to OmpA constructs<br>Michał K.</h3> |
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<li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing">dephosphorylation (with CIAP)</a> of pACYC177+OmpA_alpha with SacI and NotI. </li> | <li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing">dephosphorylation (with CIAP)</a> of pACYC177+OmpA_alpha with SacI and NotI. </li> | ||
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<li> Transformation of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top">TOP10</a> strain with ligation. </li> | <li> Transformation of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top">TOP10</a> strain with ligation. </li> | ||
<li> Transformants plating on LB + kanamycin. </li> | <li> Transformants plating on LB + kanamycin. </li> | ||
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<h3>Cloning of protein A DNA to geneart plasmid in place of protein Z DNA<br>Antoni</h3> | <h3>Cloning of protein A DNA to geneart plasmid in place of protein Z DNA<br>Antoni</h3> | ||
<li>Inoculated to liquid LB + ampicylin pGeneart+Z i pKS+A</li> | <li>Inoculated to liquid LB + ampicylin pGeneart+Z i pKS+A</li> | ||
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Revision as of 21:30, 1 October 2008
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Cloning of protein Z DNA to OmpA constructs
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