Team:Warsaw/Calendar-Main/14 October 2008

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Preparation of pSB2K3 + linker_alpha (BBa_K103009)

Piotr

  1. Transformation of TOP10 with overnight ligation - pSB2K3 + linker_alpha (BBa_K103009).
  2. Tranformants plating on LB with kanamycin.

Preparation of pACYC177 + OmpA_omega_

Michał K.

  1. Isolation of plasmid from culture inoculated on previous day - pACYC177 + OmpA_omega_.
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - still no proper clones found.

Preparation of BioBricks

Michał K.

  1. Isolation of plasmid from culture inoculated on previous day (5kb (??????) + pT7_omega_link, RFP(?????) + pT7_alpha and 3kb (??????) + OmpA_omega_link).
  2. Control digest of isolated plasmids (5kb (??????) + pT7_omega_link and 3kb (??????) + OmpA_omega_link) with EcoRI and PstI (Orange buffer). Gel electrophoresis - still no proper clones found for 3kb (??????) + OmpA_omega_link ligation but we found good clones for (5kb (??????) + pT7_omega_link.

Piotr

  1. Transformation of TOP10 with overnight ligations.
  2. Tranformants planting on LB with kanamycin - 3kb(?????)+ link_alpha, 3kb(????)_pLac_OmpA_ + alpha2 and RFP(?????) + AraC+pBAD+AID on LB with ampicillin.