Team:Warsaw/Calendar-Main/14 October 2008

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Preparation of linker_alpha (BBa_K103009)

Piotr

  1. Transformation of TOP10 with overnight ligation - pSB2K3 + linker_alpha (BBa_K103009).
  2. Tranformants plating on LB with kanamycin.

Preparation of OmpA-linker-omega-linker (BBa_K103016)

Michał K.

  1. Isolation of plasmid from culture inoculated on previous day - pACYC177 + OmpA-linker-omega-linker (BBa_K103016).
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - still no proper clones found.

Preparation of alpha_linker under PT7 (BBa_K103019)

Michał K.

Isolation of plasmid from culture inoculated on previous day - pSB1A3 carrying alpha_linker under PT7 (BBa_K103019).

Preparation of omega_linker under PT7 (BBa_K103020)

Michał K.

  1. Isolation of plasmid from culture inoculated on previous day (pSB2K3 + omega_linker under PT7 (BBa_K103020)).
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.

Preparation of AID under pBAD/araC (BBa_K103002)

Michał K.

  1. Transformation of TOP10 with overnight ligation - pSB1A3 + AraC+pBAD+AID.
  2. Tranformants plating on LB with ampicillin.

Preparation of OmpA_linker_alpha_linker under Plac

Piotr

  1. Transformation of TOP10 with overnight ligation - pSB2K3_pLac_OmpA_ + alpha2.
  2. Tranformants plating on LB with kanamycin.