Team:Warsaw/Calendar-Main/15 May 2008

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PCRs for fusions

Michał K.

  1. PCR - AID for translational fusion.
    Primers: AIDlNrH AIDpLinB
    Template DNA: pTrc99A-AID
    Annealing temperature: 55°C
    Elongation time: 60 s
    20 cycles
  2. PCR - T7 RNA-polymerase for translational fusion; Taq polymerase with supplemented buffer (Łukasz Banasiak helped us with that problematic PCR).
    Primers: T7lLinkB T7pXbSal
    Template DNA: E. coli Rosetta genomic DNA
    Annealing temperature: 62°C
    Elongation time: 4 minutes
    20 cycles
  3. PCR - T7 RNA-polymerase for transcriptional fusion.
    Primers: T7lRBSHi T7pXbSal
    Template DNA: E. coli Rosetta genomic DNA
    Annealing temperature: 62°C
    Elongation time: 4 minutes
    20 cycles