Team:Warsaw/Calendar-Main/18 June 2008

(Difference between revisions)

Revision as of 00:51, 12 October 2008


	Attribution analyzer / Interactive list of topics

Isolation of pACYC177.
Digest of purified pACYC177 with NdeI and BamHI (Tango 2x buffer).
Gel electrophoresis and isolation of proper band (3200 bp).
Clean-up of OmpA_alpha and OmpA_omega digest products (Fig. 1).
Ligation of purified DNA (pACYC177 with OmpA_alpha and OmpA_omega DNA fragments).
Transformation of E.coli TOP10 with ligation products.
Plating transformants on LB+tetracycline.

Fig. 1. OmpA_alpha (lane 2) and OmpA_omega (lane 3) digest products.

Counting of blue colonies:

Results of rifampicin test
Strain	Inducer / repressor	Number of colonies on rifampicin
Top10	none	8
Top10 pMPM-AID	0.1% Arabinose	72
Top10 pMPM-AID	none	4
Top10 pMPM-A+T13	0.1% Arabinose	148
Top10 pMPM-A+T13	none	6
Top10 pMPM-AT6	0.1% Arabinose	21
Top10 pMPM-AT6	none	27

@@ Line 2: / Line 2: @@
 <!-- do not edit above me! -->
 <html>
 <h3>Preparation of constructs with OmpA protein fusions</h3>
 <h4>Michał K.</h4>
@@ Line 11: / Line 12: @@
 <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <i>E.coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> with ligation products.</li>
 <li>Plating transformants on LB+tetracycline.</li></ol>
+<a name="fig1"> <img src="https://static.igem.org/mediawiki/2008/c/c9/Ompa_Alpha_Omega_Cleanup_WAW.jpg" width=300/> </a>
+<var> Fig. 1. OmpA_alpha (lane 2) and OmpA_omega (lane 3) digest products.</var>
 <h3> Blue/white and rifampicin test </h3>
@@ Line 38: / Line 42: @@
 <tr><th>Top10 pMPM-AT6</th><td>none</td><td>27</td></tr>
 </table>
-<a name="fig1"> <img src="https://static.igem.org/mediawiki/2008/c/c9/Ompa_Alpha_Omega_Cleanup_WAW.jpg" width=300/> </a>
-<var> Fig. 1. OmpA_alpha (lane 2) and OmpA_omega(lane 3) digest products.</var>
 </html>
 <!-- do not remove this! -->
 {{WarNotebookEnd}}