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From 2008.igem.org

Attribution analyzer / Interactive list of topics   Preparation of constructs with OmpA protein fusions Michał K. Inoculation of separate transformant colonies from plates with ligations: pACYC177+OmpA_alpha pACYC177+OmpA_omega (liquid LB + kanamycin). There is no mutation in beta-galactosidase gene. Why the colonys are white??? The analysis of lacZ sequence from pZC There is no mutation in beta-galactosidase gene. Why the colonys are white??? Colony PCR. Template: DNA isolated from white colonies Primers: pZCseqL and pZCseqR Colony PCR program Temperature Time No. of cycles 94°C 4:00 94°C 0:30 28 cycles 48°C 0:45 72°C 1:30 72°C 10:00 4°C infinite

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