Team:Warsaw/Calendar-Main/19 September 2008

From 2008.igem.org

(Difference between revisions)
Line 5: Line 5:
<html>
<html>
 +
 +
<h3>'Hunter and prey' system tests: Competition tests</h3>
 +
<h4>Weronika</h4>
 +
 +
 +
<p>
 +
Inoculation to one tube (0.25 mM IPTG; ampicillin 50 μl/ml)           
 +
<ul><li>Omp_A_omega, Omp_omega and Omp_Z_omega - A_alpha protein added ~100 μg (</li>
 +
<li>Omp_A_omega, Omp_omega and Omp_Z_omega - Z_alfa protein added ~100 μg</li>
 +
<li>Omp_A_alpha, Omp_alpha and Omp_Z_alpha - Z_omega protein added ~100 μg</li> </ul></p>
 +
 +
 +
<h3>Preparation of BioBricks</h3>
<h3>Preparation of BioBricks</h3>
<h4>Michał K.</h4>
<h4>Michał K.</h4>

Revision as of 10:57, 25 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 



'Hunter and prey' system tests: Competition tests

Weronika

Inoculation to one tube (0.25 mM IPTG; ampicillin 50 μl/ml)

  • Omp_A_omega, Omp_omega and Omp_Z_omega - A_alpha protein added ~100 μg (
  • Omp_A_omega, Omp_omega and Omp_Z_omega - Z_alfa protein added ~100 μg
  • Omp_A_alpha, Omp_alpha and Omp_Z_alpha - Z_omega protein added ~100 μg

Preparation of BioBricks

Michał K.

  1. Gel electrophoresis to estimate concentration of purified DNA from previous day.
  2. Ligation of isolated DNA fragments of RFP(??????) and deltaA.

Piotr

  1. E. coli TOP10 transformation with the above ligation.
  2. Plating on LB + ampicillin.

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/19_September_2008"