Team:Warsaw/Calendar-Main/20 May 2008

From 2008.igem.org

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<h4>Michał K: </h4>
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<p>Michał K: <br>
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1.<html><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - translation fusion: AID + T7 RNA-polymerase - optimization (temperature gradient 60&deg;C - 80&deg;C).</p>
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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - translation fusion: AID + T7 RNA-polymerase - optimization (temperature gradient 60&deg;C - 80&deg;C).</p>
<p>Primers: </p>
<p>Primers: </p>
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDlNrH">AIDlNrH</a> and  
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDlNrH">AIDlNrH</a> and  
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a></html> <br>
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a> <br>
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35 cycles <br>
35 cycles <br>
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2. Optimization of <html><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - translation fusion: AID + T7 RNA-polymerase - MgCl2 cocentration and number of cycles.
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<li> Optimization of <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - translation fusion: AID + T7 RNA-polymerase - MgCl<sub>2</sub> cocentration and number of cycles.
<p>Primers: </p>
<p>Primers: </p>
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<p><html>
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<p>
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDlNrH">AIDlNrH</a> and  
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDlNrH">AIDlNrH</a> and  
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a></html></p>
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a></p>
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Elongation temperature: 73&deg;C<br>
Elongation temperature: 73&deg;C<br>
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Annealing time: 4 minutes <br>
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Elongation time: 4 minutes <br>
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</li>
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3. Gel electrophoresis of PCR products.
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<li> Gel electrophoresis of PCR products.</li>
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</ol></p>
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Michał L., Ewa, Marcin:
 
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Plating colonies from the previous day on rifampicin
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<h4>Michał L., Ewa, Marcin:</h4>
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<p>Plating colonies from the previous day on rifampicin</p>
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Revision as of 18:40, 1 October 2008

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Michał K:

  1. PCR - translation fusion: AID + T7 RNA-polymerase - optimization (temperature gradient 60°C - 80°C).

    Primers:

    AIDlNrH and T7pXbSal
    Template DNA: purified PCR products from 16 May - AID and T7 RNA-polymerase for translation fusion
    Elongation time: 4 minutes
    35 cycles

  2. Optimization of PCR - translation fusion: AID + T7 RNA-polymerase - MgCl2 cocentration and number of cycles.

    Primers:

    AIDlNrH and T7pXbSal

    Template DNA: purified PCR products from 16 May - AID and T7 RNA-polymerase for translation fusion
    Elongation temperature: 73°C
    Elongation time: 4 minutes
  3. Gel electrophoresis of PCR products.

Michał L., Ewa, Marcin:

Plating colonies from the previous day on rifampicin

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