Team:Warsaw/Calendar-Main/20 May 2008

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(Difference between revisions)
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Annealing time: 4 minutes
Annealing time: 4 minutes
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20 cycles
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35 cycles
3. Gel electrophoresis and DNA isolation from proper band (translation fusion: AID + T7 RNA-polymerase)  
3. Gel electrophoresis and DNA isolation from proper band (translation fusion: AID + T7 RNA-polymerase)  

Revision as of 21:18, 30 September 2008

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Michał K:
1. PCR - translation fusion: AID + T7 RNA-polymerase - optymalization (temperature gradient 60°C - 80°C).

Primers:

AIDlNrH and T7pXbSal

Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion Annealing temperature: 55 °C Annealing time: 4 minutes 35 cycles 3. Gel electrophoresis and DNA isolation from proper band (translation fusion: AID + T7 RNA-polymerase)