Team:Warsaw/Calendar-Main/20 May 2008

From 2008.igem.org

Revision as of 21:05, 30 September 2008 by MKrzyszton (Talk | contribs)

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 



Michał K:
1. PCR - translation fusion: AID + T7 RNA-polymerase

Primers:

AIDlNrH and T7pXbSal

Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion Annealing temperature: 55 °C Annealing time: 4 minutes 20 cycles 3. Gel electrophoresis and DNA isolation from proper band (translation fusion: AID + T7 RNA-polymerase)



Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/20_May_2008"