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Team:Warsaw/Calendar-Main/20 May 2008
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Revision as of 11:18, 5 October 2008 by
MKrzyszton
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Attribution analyzer
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Interactive list of topics
Michał K.:
Digest
of PCR product for transcription fusion (T7 RNA-polymerase) with HindIII and SalI (2x Tango buffer).
Digest
of PCR product for translation fusion (AID+T7 RNA-polymerase) with NcoI and XbaI (1x Tango buffer).
Clean-up
of digested products from p.1 and p.2.
Digest
of pMPM-T5+AID with HindIII and SalI (2x Tango buffer).
Digest
of
pMPM-T5
with NcoI and XbaI (1x Tango buffer).
Gel-electrophresis and
gel-out
of proper bands (p.4 - 4850 bp, p.5 - 4250 bp).
Ligation
of purified products from p.1 and p.4.
Electroporation
of
E. coli
TOP10
with ligation product.
Ligation
of digested products from p.4 and p.5.
Electroporation
of
E. coli
TOP10
with ligation product.
Transformants plating on LB + tetracycline.
Michał L., Ewa, Marcin:
Plating colonies from the previous day on rifampicin.
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